DNA damage effect of cyprodinil and thiacloprid in adult zebrafish gills


Toğay V. A., Yavuz Türel G., Aşcı Çelik D., Özgöçmen M., Evgen Tuluceoglu E., Sen I., ...More

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, vol.28, no.12, pp.14482-14487, 2021 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 28 Issue: 12
  • Publication Date: 2021
  • Doi Number: 10.1007/s11356-020-11668-1
  • Journal Name: ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, IBZ Online, ABI/INFORM, Aerospace Database, Agricultural & Environmental Science Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, EMBASE, Environment Index, Geobase, MEDLINE, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Page Numbers: pp.14482-14487
  • Keywords: Comet assay, Cyprodinil, DNA damage, Thiacloprid, Zebrafish, OXIDATIVE STRESS, NEONICOTINOID INSECTICIDES, FLUDIOXONIL PESTICIDES, TOXICITY, EXPOSURE, DELTAMETHRIN
  • Süleyman Demirel University Affiliated: Yes

Abstract

Cyprodinil and thiacloprid are two of the most commonly used pesticides in Turkey. It is more likely to reach humans or animals due to their widespread use. This study aims to investigate whether there is a DNA damage risk due to cyprodinil and thiacloprid exposure. Zebrafish, which is used as a model organism in health and environmental research, and comet assay were chosen to demonstrate this damage. Ten zebrafish per group were exposed to 2 different concentrations for each pesticides (0.31 and 0.155 mg/L for cyprodinil and 1.64 and 0.82 mg/L for thiacloprid) for 21 days. After, gills were excised and comet assay was performed. Photos of an average of 50 cells per slide were taken and were analyzed with visual evaluation program. DNA damage was found to be increased in the 0.31 mg/L cyprodinil, 0.82 mg/L thiacloprid, and 1.64 mg/L thiacloprid treatment groups when compared to the control group (p < 0.001). Average tail DNA percentage parameter values were 9.45 +/- 0.51, 10.30 +/- 0.34, 11.17 +/- 0.33, and 2.47 +/- 0.06 respectively. Cyprodinil and thiacloprid were identified as genotoxic agents that should be investigated further.