© 2021This study aimed to determine the phytochemical content of palmarosa (Cymbopogon martini) essential oil and tested for its antibacterial and anti-quorum sensing activity. The possible inhibition effect by molecular modeling was also revealed. It was recorded that geraniol (81.25%) is the main component of palmarosa essential oil and found effective against Gram-positive and Gram-negative bacteria. The essential oil was found to reduce pyocyanin production (by 71%), elastase activity (by 64%), swarming motility (by 69%), and biofilm formation (by 64%). Furthermore, geraniol was docked against the binding region of the LasR protein and found to excellently bound with the receptor. These results showed that palmarosa essential oil acts as a green solution by exerting potential antibacterial activity against most bacteria and has a significant anti-quorum sensing effect on Pseudomonas aeruginosa PAO1 via geraniol.